| 质粒名称: | ilux pGEX(-) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 107879 |
| 存储实验室: | Stefan Hell |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX(-) |
| 空载体大小: | 4268 bp |
| 完整质粒大小: | 10873 bp |
| 载体修饰: | GST删除版pGEX-6P-1 |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ilux |
| 物种来源: | Synthetic |
| 插入大小: | 6605 bp |
| 启动子: | tac |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CGGTTCTGGCAAATATTCTGAA |
| 3’测序引物 | CATGTGTCAGAGGTTTTCAC |
|
文献引用方法 |
|
| 材料和方法部分: | ilux pGEX(-) was a gift from Stefan Hell (Addgene plasmid # 107879 ; http://n2t.net/addgene:107879 ; RRID:Addgene_107879) |
| 参考文献部分: | Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Gregor C, Gwosch KC, Sahl SJ, Hell SW. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. 10.1073/pnas.1715946115 PubMed 29339494 |
买家导航
ilux pGEX(-)载体 iluxpGEX(-)质粒基本信息
ilux pGEX(-)质粒图谱 iluxpGEX(-)载体图谱和ilux pGEX(-)载体序列 iluxpGEX(-)质粒序列多克隆位点信息
质粒图谱.png)
ilux pGEX(-)质粒 iluxpGEX(-)载体简介
ilux pGEX(-)质粒序列 iluxpGEX(-)载体序列
LOCUS ilux pGEX(-) 10873 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Bacterial expression of ilux for bioluminescence imaging.
ACCESSION .
VERSION .
KEYWORDS ilux pGEX(-)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10873)
AUTHORS Gregor C, Gwosch KC, Sahl SJ, Hell SW
TITLE Strongly enhanced bacterial bioluminescence with the ilux operon for
single-cell imaging.
JOURNAL Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi:
10.1073/pnas.1715946115. Epub 2018 Jan 16.
PUBMED 29339494
REFERENCE 2 (bases 1 to 10873)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10873
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 44..72
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 80..96
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 119..1561
/codon_start=1
/gene="luxC"
/product="LuxC fatty acid reductase"
/label=LuxC
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTKKISFIITGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
IIDCNGNNELRLHDIVNFLYTVGQRWKNDEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGPKKSLCIIDNPVDLTSAATGAAHDVC
FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
FAGLKVEVDIHQRWTIIESDAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
DVAVEIEQTRFLEEDKFLVFVP"
CDS 1574..2497
/codon_start=1
/gene="luxD"
/product="LuxD acyltransferase"
/label=LuxD
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
FEHLTIATVNERRMRIEIENQAISLS"
CDS 2552..3634
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/label=LuxA
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMERLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGADMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESAATTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
CDS 3649..4632
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/label=LuxB
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGLFFLNFINPTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
FSDCEKKDEMHFFNRPAEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNEDFENKLEEIIAENAVGNYTECIT
AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
CDS 4817..5929
/codon_start=1
/gene="luxE"
/product="LuxE"
/label=LuxE
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
primer_bind complement(6784..6806)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6951..6969)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7037..7141
/gene="bla"
/label=AmpR promoter
CDS 7142..8002
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7360..7379)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8173..8761
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8662..8681
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8915..8932
/label=L4440
/note="L4440 vector, forward primer"
promoter 9005..9082
/gene="lacI"
/label=lacI promoter
CDS 9083..10165
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind complement(9102..9121)
/label=LacI-R
/note="LacI, reverse primer"
promoter 10214..10244
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10252..10268
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 10276..10292
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 10276..10292
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 10288..10815
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
QPSEAVVWLCRS"
primer_bind complement(10308..10325)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(10308..10324)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10317..10339)
/label=M13/pUC Forward
/note="In lacZ gene"
ORIGIN
1 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg
61 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa caggatccat
121 gactaaaaaa atttcattca ttattaccgg ccaggttgaa atctttcccg aaagcgatga
181 tttagtgcaa tccattaatt ttggtgataa tagtgtttac ctgccaatat tgaatgactc
241 tcatgtaaaa aacattattg attgtaatgg aaataacgaa ttacggttgc atgacattgt
301 caattttctc tatacggtag ggcaaagatg gaaaaatgat gaatactcaa gacgcaggac
361 atacattcgt gacttaaaaa aatatatggg atattcagaa gaaatggcta agctagaggc
421 caattggata tctatgatat tatgttctaa aggcggcctt tatgatgttg tagaaaatga
481 acttggttct cgccatatca tggatgaatg gctacctcag gatgaaagtt atgttcgggc
541 ttttccgaaa ggtaaatctg tacatctgtt ggcaggtaat gttccattat ctgggatcat
601 gtctatatta cgcgcaattt taactaagaa tcagtgtatt ataaaaacat cgtcaaccga
661 tccttttacc gctaatgcat tagcgttaag ttttattgat gtagacccta atcatccgat
721 aacgcgctct ttatctgtta tatattggcc ccaccaaggt gatacatcac tcgcaaaaga
781 aattatgcga catgcggatg ttattgtcgc ttggggaggg ccagatgcga ttaattgggc
841 ggtagagcat gcgccatctt atgctgatgt gattaaattt ggtcctaaaa agagtctttg
901 cattatcgat aatcctgttg atttgacgtc tgcagcgaca ggtgcggctc atgatgtttg
961 tttttacgat cagcgagctt gtttttctgc ccaaaacata tattatatgg gaaatcatta
1021 tgaggaattt aagttagcgt tgatagaaaa acttaatcta tatgcgcata tattaccgaa
1081 tgcaaaaaaa gattttgatg aaaaggcggc ctattcttta gttcaaaaag aaagcttgtt
1141 tgctggatta aaagtagagg tggatattca tcaacgttgg acgattattg agtcagatgc
1201 aggtgtggaa tttaatcaac cacttggcag atgtgtgtac cttcatcacg tcgataatat
1261 tgagcaaata ttgccttatg ttcaaaaaaa taagacgcaa accatatcta tttttccttg
1321 ggagtcatca tttaaatatc gagatgcgtt agcattaaaa ggtgcggaaa ggattgtaga
1381 agcaggaatg aataacatat ttcgagttgg tggatctcat gacggaatga gaccgttgca
1441 acgattagtg acatatattt ctcatgaaag gccatctaac tatacggcta aggatgttgc
1501 ggttgaaata gaacagactc gtttcctgga agaagataag ttccttgtat ttgtcccata
1561 ataggtaaaa agtatggaaa atgaatcaaa atataaaacc atcgaccacg ttatttgtgt
1621 tgaaggaaat aaaaaaattc atgtttggga aacgctgcca gaagaaaaca gcccaaagag
1681 aaagaatgcc attattattg cgtctggttt tgcccgcagg atggatcatt ttgctggtct
1741 ggcggaatat ttatcgcgga atggatttca tgtgatccgc tatgattcgc ttcaccacgt
1801 tggattgagt tcagggacaa ttgatgaatt tacaatgtct ataggaaagc agagcttgtt
1861 agcagtggtt gattggttaa ctacacgaaa aataaataac ttcggtatgt tggcttcaag
1921 cttatctgcg cggatagctt atgcaagcct atctgaaatc aatgcttcgt ttttaatcac
1981 cgcagtcggt gttgttaact taagatattc tcttgaaaga gctttagggt ttgattatct
2041 cagtctaccc attaatgaat tgccgaataa tctagatttt gaaggccata aattgggtgc
2101 tgaagtcttt gcgagagatt gtcttgattt tggttgggaa gatttagctt ctacaattaa
2161 taacatgatg tatcttgata taccgtttat tgcttttact gcaaataacg ataattgggt
2221 caagcaagat gaagttatca cattgttatc aaatattcgt agtaatcgat gcaagatata
2281 ttctttgtta ggaagttcgc atgacttgag tgaaaattta gtggtcctgc gcaattttta
2341 tcaatcggtt acgaaagccg ctatcgcgat ggataatgat catctggata ttgatgttga
2401 tattactgaa ccgtcatttg aacatttaac tattgcgaca gtcaatgaac gccgaatgag
2461 aattgagatt gaaaatcaag caatttctct gtcttaaaat ctattgagat attctatcac
2521 tcaaatagca atataaggac tctctgaatt catgaaattt ggaaactttt tacttacata
2581 ccaacctccc caattttctc aaacagaggt aatggaacgt ttggttaaat taggtcgcat
2641 ctctgaggag tgtggttttg ataccgtatg gttactggag catcatttca cggagtttgg
2701 tctacttggt aacccttatg tcgctgctgc atatttactt ggcgcgacta aaaaattgaa
2761 tgtaggaacc gccgctattg ttcttcccac agcccatcca gtacgccaac ttgaagatgt
2821 gaatttattg gatcaaatgt caaaaggacg atttcggttt ggtatttgcc gagggcttta
2881 caacaaggac tttcgcgtat tcggcgcgga tatgaataac agtcgcgcct tagcggaatg
2941 ctggtacggg ctgataaaga atggcatgac agagggatat atggaagctg ataatgaaca
3001 tatcaagttc cataaggtaa aagtaaaccc cgcggcgtat agcagaggtg gcgcaccggt
3061 ttatgtggtg gctgaatcag ctgcgacgac tgagtgggca gctcaatttg gcctaccgat
3121 gatattaagt tggattataa atactaacga aaagaaagca caacttgagc tttataatga
3181 ggtggctcaa gaatatgggc acgatattca taatatcgac cattgcttat catatataac
3241 atctgtagat catgactcaa ttaaagcgaa agagatttgc cggaaatttc tggggcattg
3301 gtatgattct tatgtgaatg ctacgactat ttttgatgat tcagaccaaa caagaggtta
3361 tgatttcaat aaagggcagt ggcgtgactt tgtattaaaa ggacataaag atactaatcg
3421 ccgtattgat tacagttacg aaatcaatcc cgtgggaacg ccgcaggaat gtattgacat
3481 aattcaaaaa gacattgatg ctacaggaat atcaaatatt tgttgtggat ttgaagctaa
3541 tggaacagta gacgaaatta ttgcttccat gaagctcttc cagtctgatg tcatgccatt
3601 tcttaaagaa aaacaacgtt cgctattata ttagctaagg agaaagaaat gaaatttgga
3661 ttgttcttcc ttaacttcat caatccgaca actgttcaag aacaaagtat agttcgcatg
3721 caggaaataa cggagtatgt tgataagttg aattttgaac agattttagt gtatgaaaat
3781 catttttcag ataatggtgt tgtcggcgct cctctgactg tttctggttt tctgctcggt
3841 ttaacagaga aaattaaaat tggttcatta aatcacatca ttacaactca tcatcctgtc
3901 cgcatagcgg aggaagcttg cttattggat cagttaagtg aagggagatt tattttaggg
3961 tttagtgatt gcgaaaaaaa agatgaaatg cattttttta atcgcccggc tgaatatcaa
4021 cagcaactat ttgaagagtg ttatgaaatc attaacgatg ctttaacaac aggctattgt
4081 aatccagata acgattttta tagcttccct aaaatatctg taaatcccca tgcttatacg
4141 ccaggcggac ctcggaaata tgtaacagca accagtcatc atattgttga gtgggcggcc
4201 aaaaaaggta ttcctctcat ctttaagtgg gatgattcta atgatgttag atatgaatat
4261 gctgaaagat ataaagccgt cgcggataaa tatgacgttg acctatcaga gatagaccat
4321 cagttaatga tattagttaa ctataacgaa gatagtaata aagctaaaca agagacgcgt
4381 gcatttatta gtgattatgt tcttgaaatg caccctaatg aagatttcga aaataaactt
4441 gaagaaataa ttgcagaaaa cgctgtcgga aattatacgg agtgtataac tgcggctaaa
4501 ttggcaattg aaaagtgtgg tgcgaaaagt gtattgctgt cctttgaacc aatgaatgat
4561 ttgatgagcc aaaaaaatgt aatcaatatt gttgatgata atattaagaa gtaccacatg
4621 gaatatacct aaccatggta gatttcgagt tgcagcgagg cggcaagtga acgaatcccc
4681 aggagcatag ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg
4741 aaagatgaag ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt
4801 gaggagtaaa acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga
4861 aattgatgat ttgatttttt cgagcgatcc attagtgtgg tcttacgacg agcaggaaaa
4921 aatcagaaag aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt gtcgagaata
4981 tcgtcactac tgtcaggcac acaaagtaga tgacaatatt acggaaattg atgacatacc
5041 tgtattccca acatcggttt ttaagtttac tcgcttatta acttctcagg aaaacgagat
5101 tgaaagttgg tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg cgcgtgacag
5161 attaagtatt gagagactct taggctctgt gagttatggc atgaaatatg ttggtagttg
5221 gtttgatcat caaatagaat tagtcaattt gggaccagat agatttaatg ctcataatat
5281 ttggtttaaa tatgttatga gtttggtgga attgttatat cctacgacat ttaccgtaac
5341 agaagaacga atagattttg ttaaaacatt gaatagtctt gaacgaataa aaaatcaagg
5401 gaaagatctt tgtcttattg gttcgccata ctttatttat ttactctgcc attatatgaa
5461 agataaaaaa atctcatttt ctggagataa aagcctttat atcataaccg gaggcggctg
5521 gaaaagttac gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt tatttgatac
5581 tttcaatctc agtgatatta gtcagatccg agatatattt aatcaagttg aactcaacac
5641 ttgtttcttt gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat atgcgcgagc
5701 gcttgatcct gaaacgttga aacctgtacc tgatggaacg ccggggttga tgagttatat
5761 ggatgcgtca gcaaccagtt atccagcatt tattgttacc gatgatgtcg ggataattag
5821 cagagaatat ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg tcaatacgag
5881 gacgcagaaa gggtgtgctt taagcttaac cgaagcgttt gatagttgag tcgacctaag
5941 gagaaagaaa tggtgaagat acagcccatc cccacaacta gccagggcag cctttttata
6001 atgaatagca ccatagagac aatcctgggc catagatcca ttaggaagtt cacatctgaa
6061 cctattgcta gtgagcagct gcaaacgatt cttcagtctg ggctcgctgc ttcaagctca
6121 tccatgctgc aggttgtgag tataattcgg gttacagaca cggaaaagag aaaattgctc
6181 gctcaatatg ccggcaacca gacgtatgtg gaatccgctg ctgagttcct ggtcttttgt
6241 atagactacc agcgacacgc tactatcaac cccgatgtcc aagctgactt taccgagctg
6301 accctgattg gtgcagtgga ttccggcata atggcccaga attgcctcct ggcagcagaa
6361 tcaatgggtc ttggcggagt ctatatcgga ggacttcgga actcagctgc ccaagtggat
6421 gagttgctcg gactgcccaa gaacacagct atcctcttcg gaatgtgctt ggggcacccc
6481 gatcagagcc ctgagacaaa gcctagactg cccgctcatg tgatcgtgca cgagaaccaa
6541 tatcaagctc tgaacattga cgacgtacag gcgtatgaca aaacattgca ggagtattat
6601 gccagcagaa ccagcaacca gaagcagagt gtctggtccc aggaaactgc aggcaagctg
6661 gccggagaat ccctcccaca catcctgcca tacctgaact ccaagggcct tgccagaagg
6721 taagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg atgacggtga
6781 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
6841 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
6901 gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg gcctcgtgat
6961 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
7021 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
7081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
7141 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
7201 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
7261 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
7321 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
7381 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
7441 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
7501 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
7561 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
7621 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
7681 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
7741 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
7801 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
7861 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
7921 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
7981 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
8041 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
8101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
8161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
8221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
8281 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
8341 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
8401 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
8461 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
8521 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
8581 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
8641 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
8701 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
8761 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
8821 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
8881 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
8941 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataaa
9001 ttccgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
9061 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
9121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
9181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
9241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
9301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
9361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
9421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
9481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
9541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
9601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
9661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
9721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
9781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
9841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
9901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
9961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
10021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccacgc tagcgcccaa
10081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
10141 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag ctcactcatt
10201 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
10261 gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt
10321 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
10381 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
10441 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
10501 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
10561 cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat
10621 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
10681 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg aattagctta
10741 tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc tgtggtatgg
10801 ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca aggcgcactc ccgttctgga
10861 taatgttttt tgc
//
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